| Autor: |
ND. Tenakanai, J.K. Banamu, Y. Lin, D. Walsh, R. Simbil, M. Globan, A. Penn-Nicholson, P. du Cros, J. Greig |
| Jazyk: |
angličtina |
| Rok vydání: |
2024 |
| Předmět: |
|
| Zdroj: |
Public Health Action, Vol 14, Iss 4, Pp 152-157 (2024) |
| Druh dokumentu: |
article |
| ISSN: |
2220-8372 |
| DOI: |
10.5588/pha.24.0029 |
| Popis: |
SETTING: Papua New Guinea is a high-burden multidrug-resistant TB (MDR/RR-TB) country that reports low rates of bacteriological confirmation. Sputum drug susceptibility testing for second-line drugs is important. Access to culture is limited. OBJECTIVE: To evaluate the prevalence of mycobacterial sputum sample culture contamination and determine factors associated with contamination. DESIGN: A retrospective analysis of data from a TB diagnostic accuracy study that used culture as the diagnostic reference standard. Data included characteristics of people with presumptive pulmonary TB who provided sputum, sputum quality and culture results. RESULTS: Sputum (1–3 samples) was collected from 174 adults. The median age was 33 years (IQR 24–47). Of 337 samples sent for culture, 28% were contaminated. Contamination was strongly associated with samples self-collected by participants outside clinic hours (aOR 5.69; 95% CI 2.62–12.38). Contamination risk increased with delays in shipping to the reference laboratory (aOR 1.19 per day, 95% CI 1.10–1.29) beyond the minimum 3 days. Contamination was less frequent among people aged 35–44 years compared to 18–24 years (aOR 0.27, 95% CI 0.10–0.73). Sputum quality was not associated with culture contamination. CONCLUSION: Culture contamination could be reduced using spot sputum collection, expedited submission to laboratories and faster shipping when required. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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