DNA content of Spermatozoa with respect to Seminal Sperm Concentration using a Microchemical Spectrophotometric Method
| Autor: | Munaf S. Daoud, Faraid A. AL- chalabi |
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| Jazyk: | angličtina |
| Rok vydání: | 2007 |
| Předmět: | |
| Zdroj: | مجلة كلية الطب, Vol 48, Iss 4 (2007) |
| Druh dokumentu: | article |
| ISSN: | 0041-9419 2410-8057 |
| Popis: | Background: To determine the DNA content in subfertile patients and to correlate it with seminal sperm concentration. Design: Prospective observational study. Setting: College of Medicine, Dept. of Physiological chemistry and Institute for embryo Research and infertility treatment-University of Baghdad.The study was conducted through years 2004-2005. Methods: A random sample of 61 subfertile male patients undergoing semen evaluation and aged from 20-45 years were studied. Semen samples were assessed for seminal sperm concentration microscopically and were classified into 3 different groups according to count (million/ml).Then sperm D N A content (μg/ml) was estimated using a microchemical spectrophotometric method. Results: The three groups were statistically of significant difference( P 0.05 )correlation was observed in the other two groups (Gr. I & Gr. II ). Conclusion: Significant positive correlation was obtained between sperm concentration and DNA content. in the oligozoospermic subfertile patient , but no significant correlation was found in the normozoopermic patients . D N A content per spermatozoan from oligozoospermic patients was higher than that in normozoospermic patients. |
| Databáze: | Directory of Open Access Journals |
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