Interplay of heavy chain introns influences efficient transcript splicing and affects product quality of recombinant biotherapeutic antibodies from CHO cells

Autor:	Emma Kelsall, Claire Harris, Titash Sen, Diane Hatton, Sarah Dunn, Suzanne Gibson
Jazyk:	angličtina
Rok vydání:	2023
Předmět:	Alternative splicing CHO expression plasmid intron monoclonal antibody splice variant Therapeutics. Pharmacology RM1-950 Immunologic diseases. Allergy RC581-607
Zdroj:	mAbs, Vol 15, Iss 1 (2023)
Druh dokumentu:	article
ISSN:	19420862 1942-0870 1942-0862
DOI:	10.1080/19420862.2023.2242548
Popis:	ABSTRACTIntrons are included in genes encoding therapeutic proteins for their well-documented function of boosting expression. However, mis-splicing of introns in recombinant immunoglobulin (IgG) heavy chain (HC) transcripts can produce amino acid sequence product variants. These variants can affect product quality; therefore, purification process optimization may be needed to remove them, or if they cannot be removed, then in-depth characterization must be carried out to understand their effects on biological activity. In this study, HC transgene engineering approaches were investigated and were successful in significantly reducing the previously identified IgG HC splice variants to
Databáze:	Directory of Open Access Journals
Externí odkaz:	https://doaj.org/article/ab876963243a4450ba80756faf129466 Zobrazit plný text záznamu View record in DOAJ