Functional characteristic and differential expression of myostatin in Chlamys nobilis

Autor: Kecheng Zhu, Huayang Guo, Nan Zhang, Yundong Li, Shigui Jiang, Dianchang Zhang
Jazyk: angličtina
Rok vydání: 2018
Předmět:
Zdroj: Journal of Applied Animal Research, Vol 46, Iss 1, Pp 685-690 (2018)
Druh dokumentu: article
ISSN: 0971-2119
0974-1844
09712119
DOI: 10.1080/09712119.2017.1380646
Popis: Myostatin (MSTN) was a conserved negative regulatory protein of muscle growth and development in numerous species. In this study, the MSTN gene was cloned and characterized from a noble scallop, Chlamys nobilis, and it was named CN-MSTN. To investigate the molecular characterization of MSTN and its gene expression profile of spatio-temporal, we isolated the MSTN cDNA sequence in C. nobilis and analysed expression patterns using quantitative real-time PCR. CN-MSTN cDNA contained a 1,374 bp open reading frame that encoded a 458 amino acids. Furthermore, the result of 3D model indicated that CN-MSTN mature peptide was similar to that of Chlamys farreri. Additionally, based on both the nucleotide and amino acid datasets, phylogenetic trees indicated that C. nobilis has the highest similarity with homologues of C. farreri. The expression patterns of different stages showed that MSTN expression was markedly higher in the gastrulae period. Additionally, remarkable expression of MSTN was observed in adductor muscle than with other tissues. Our data would provide expression and phylogenetic information of this economical important sea food, noble scallop.
Databáze: Directory of Open Access Journals
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