Half-Preparative Scale Synthesis of (S)-1-Phenylethane-1,2-Diol as a Result of 2-Phenylethanol Hydroxylation with Aspergillus niger (IAFB 2301) Assistance

Autor: Beata Szmigiel-Merena, Małgorzata Brzezińska-Rodak, Magdalena Klimek-Ochab, Paulina Majewska, Ewa Żymańczyk-Duda
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Zdroj: Symmetry, Vol 12, Iss 6, p 989 (2020)
Druh dokumentu: article
ISSN: 2073-8994
DOI: 10.3390/sym12060989
Popis: Aspergillus niger (IAFB 2301) was employed for bioconversions of 2-phenylethanol as an immobilized or free mycelium and also as a spore suspension. Experiments were conducted on laboratory and half-preparative scale (bioreactor New Brunswick Scientific, BioFlo Model C32). Thus, A. niger applied as free mycelium, depending on the outcome, supported formation of the mixture of 4-hydroxyphenylacetic acid and hydroxytyrosol (final concentration of 13.8 mg/L and 3.7% efficiency) or 4-hydroxyphenylacetic acid, as single product (final concentration of 140 mg/L and 18% efficiency). In case of scaling experiments conducted with flow and batch reactors, accordingly, the following results were achieved: 1. mixture of antioxidants 4-hydroxyphenylacetic acid and hydroxytyrosol formed with final concentration of 76 mg/L and 10% efficiency (simplified flow system and immobilized mycelium); 2. (S)-1-phenylethane-1,2-diol synthesized with a final concentration of 447 mg/L and 65% (1.3 L batch reactor).
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