Determinação de Cu, Fe, Mn, Zn e do teor de proteína total em amostras de trigo e soja após procedimento de extração sequencial
| Autor: | Leila M. Bittencourt, Diogo A. P. D. Lana, Rodinei Augusti, Letícia M. Costa, Adriano M. C. Pimenta, Agenor V. dos Santos, Ana Paula F. Gonçalves |
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| Jazyk: | English<br />Spanish; Castilian<br />Portuguese |
| Rok vydání: | 2012 |
| Předmět: | |
| Zdroj: | Química Nova, Vol 35, Iss 10, Pp 1922-1926 (2012) |
| Druh dokumentu: | article |
| ISSN: | 1678-7064 0100-4042 |
| DOI: | 10.1590/S0100-40422012001000006 |
| Popis: | A sequential extraction procedure was applied to wheat and soybean seed samples. The total protein content (determined by two distinct methods: Bradford and bicinchoninic acid-BCA) and distribution of Cu, Fe, Mn and Zn in each fraction was determined. The sequential extraction employed four different solutions: water, 0.5 mol L-1 NaCl, ethanol/water (70:30 v v-1) and 0.5 mol L-1 NaOH. For both samples, the highest concentration of metals was observed in those extracts associated with globulin-type proteins using NaCl solution. Regarding protein content, higher levels were obtained using the BCA method. |
| Databáze: | Directory of Open Access Journals |
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