| Autor: |
Svenja Pachernegg, Gorjana Robevska, Lucas G.A. Ferreira, Jocelyn A. van den Bergen, Katerina Vlahos, Sara E. Howden, Andrew H. Sinclair, Katie L. Ayers |
| Jazyk: |
angličtina |
| Rok vydání: |
2024 |
| Předmět: |
|
| Zdroj: |
Stem Cell Research, Vol 79, Iss , Pp 103494- (2024) |
| Druh dokumentu: |
article |
| ISSN: |
1873-5061 |
| DOI: |
10.1016/j.scr.2024.103494 |
| Popis: |
The transcription factor WT1 plays a critical role in several embryonic developmental processes such as gonadogenesis, nephrogenesis, and cardiac development. We generated a homozygous (MCRIi031-A-3) WT1 knockout induced pluripotent stem cell (iPSC) line from human fibroblasts using a one-step protocol for CRISPR/Cas9 gene-editing and episomal-based reprogramming. The cells exhibit a normal karyotype and morphology, express pluripotency markers, and have the capacity to differentiate into the three embryonic germ layers. These cell lines will allow us to further explore the role of WT1 in critical developmental processes. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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