In vitro conservation of Poincianella pyramidalis (Tul.) L.P. Queiroz under minimal growth conditions

Autor: Tecla dos Santos Silva, Cristina Ferreira Nepomuceno, Taliane Leila Soares, José Raniere Ferreira de Santana
Jazyk: English<br />Spanish; Castilian<br />Portuguese
Rok vydání: 2019
Předmět:
Zdroj: Ciência e Agrotecnologia, Vol 43 (2019)
Druh dokumentu: article
ISSN: 1981-1829
1413-7054
DOI: 10.1590/1413-7054201943014519
Popis: ABSTRACT Poincianella pyramidalis (Tul.) L.P. Queiroz, is an arboreal species endemic to Brazil’s Caatinga biome and an important source of lumber and also of medicinal substances. This study aimed to evaluate the effect of the osmotic agents sucrose, sorbitol and mannitol and the growth retardant paclobutrazol (PBZ) on the induction of slow growth in vitro of P. pyramidalis, seeking to establish alternative techniques for ex situ conservation of the species. In the first experiment, plantlets with seven days of age were inoculated on Woody Plant Medium (WPM) supplemented with four concentrations of sucrose (87.64, 131.46, 175.28 or 219.10 mM) combined with 0.0 or 87.64 mM sorbitol or mannitol. In the second experiment, these plantlets were inoculated on WPM medium with 0.0, 1.5, 3.0, 4.5 or 6.0 µM PBZ and supplemented with 87.64 mM sucrose. For both experiments, the survival percentage of the plants was evaluated every 60 days, and at the end of 240 days, the following parameters were recorded: number of green leaves and senescent leaves; length of the aerial part and longest root; and dry mass of the aerial part and roots. The best results to maintain the quality of the P. pyramidalis plants conserved in vitro were obtained on medium supplemented with 219.10 mM sucrose for up to 240 days without subculture. With respect to PBZ, the concentration of 6.0 μM can be recommended for in vitro conservation of P. pyramidalis for 240 days without the need of subculturing.
Databáze: Directory of Open Access Journals
Externí odkaz: