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Summary: The gammaherpesviruses, including Epstein-Barr virus (EBV), Kaposi's sarcoma-associated herpesvirus (KSHV), and murine gammaherpesvirus 68 (MHV68, MuHV-4, γHV68), are etiologic agents of a wide range of lymphomas and non-hematological malignancies. These viruses possess large and highly dense dsDNA genomes that feature >80 bidirectionally positioned open reading frames (ORFs). The abundance of overlapping transcripts and extensive splicing throughout these genomes have until now prohibited high throughput-based resolution of transcript structures. Here, we integrate the capabilities of long-read sequencing with the accuracy of short-read platforms to globally resolve MHV68 transcript structures using the transcript resolution through integration of multi-platform data (TRIMD) pipeline. This approach reveals highly complex features, including: (1) pervasive overlapping transcript structures; (2) transcripts containing intra-gene or trans-gene splices that yield chimeric ORFs; (3) antisense and intergenic transcripts containing ORFs; and (4) noncoding transcripts. This work sheds light on the underappreciated complexity of gammaherpesvirus transcription and provides an extensively revised annotation of the MHV68 transcriptome. : The highly dense dsDNA genomes of herpesviruses feature an abundance of overlapping transcripts and extensive splicing, greatly hindering the global identification of individual transcripts. O’Grady et al. use integrated multi-platform genomics to globally resolve transcript structures from murine gammaherpesvirus 68, providing an extensively revised genome annotation. Keywords: herpesvirus, gammaherpesvirus, transcriptome, murine gammaherpesvirus 68, murid herpesvirus 4, TRIMD, transcript resolution, transcript structure, virus, readthrough |
