| Autor: |
Roberto Pierro, Mariarosaria De Pascali, Alessandra Panattoni, Alessandro Passera, Alberto Materazzi, Luigi De Bellis, Andrea Luvisi, Piero Attilio Bianco, Fabio Quaglino |
| Jazyk: |
angličtina |
| Rok vydání: |
2022 |
| Předmět: |
|
| Zdroj: |
International Journal of Plant Biology, Vol 13, Iss 2, Pp 15-30 (2022) |
| Druh dokumentu: |
article |
| ISSN: |
2037-0164 |
| DOI: |
10.3390/ijpb13020004 |
| Popis: |
Grapevine “bois noir”, related to the presence of ‘Candidatus Phytoplasma solani’ (‘Ca. P. solani’), represents a serious threat in several vine-growing areas worldwide. In surveys conducted over two years, mild and/or moderate symptoms and lower pathogen titer were mainly associated with ‘Ca. P. solani’ strains harboring a secY gene sequence variant (secY52), whereas severe symptoms and higher titer were mainly observed in grapevines infected by phytoplasma strains carrying any one of another four variants. A comparison of amino acid sequences of the protein SecY of ‘Ca. P. solani’ strains revealed the presence of conservative and semi-conservative substitutions. The deduced three-dimensional (3D) protein analysis unveiled that one semi-conservative substitution identified in the sequence variant secY52 is responsible for a structural disordered region that probably confers a flexibility for binding to distinct molecular complexes. In fact, the other analyzed variants show an organized structure and the 3D in silico prediction allowed the identification of β-sheets. Thus, differences in symptom severity and pathogen concentration observed in grapevines infected by ‘Ca. P. solani’ strains carrying distinct secY gene sequence variants suggest a possible relationship between SecY protein structure and phytoplasma strain virulence. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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