Determinação de heparina fracionada em preparações farmacêuticas utilizando turbidimetria
| Autor: | Samantha Sant'Anna Marotta-Oliveira, Juliana Maldonado Marchetti |
|---|---|
| Jazyk: | English<br />Spanish; Castilian<br />Portuguese |
| Rok vydání: | 2011 |
| Předmět: | |
| Zdroj: | Química Nova, Vol 34, Iss 6, Pp 1063-1067 (2011) |
| Druh dokumentu: | article |
| ISSN: | 1678-7064 0100-4042 |
| DOI: | 10.1590/S0100-40422011000600025 |
| Popis: | A turbidimetric method has been used for quantification of fractionated heparin (FH) in pharmaceutical dosage. The UV detection at two wavelengths (290 and 500 nm) showed a significant increase in sensitivity of the method, specificity, and linearity to range 5.0-50.0 µg mL-1 and 50.0-200.0 µg mL-1, respectively (r < 0.99). At both wavelengths, the method was precise (inter-assay CV < 5.0%, and intra-assay CV < 3.0%), accurate (maximum deviation of ± 12%), and robust to the parameters evaluated. Turbidimetry proved to be easy, inexpensive and relatively fast. The results obtained attest to the reliability of the method. |
| Databáze: | Directory of Open Access Journals |
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