Development of single tubing-type canister for cryo-storage of bull semen and their effect on sperm motility and viability
| Autor: | Mohd Iswadi Ismail, Khairul Osman, Siti Fatimah Ibrahim, Farah Hanan Fatihah Jaafar, Nur Azianie Abd Ghani, Fazly Ann Zainalabidin, Abas Mazni Othman |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: | |
| Zdroj: | Brazilian Archives of Biology and Technology, Vol 57, Iss 2, Pp 194-199 (2014) |
| Druh dokumentu: | article |
| ISSN: | 1678-4324 1516-8913 |
| DOI: | 10.1590/S1516-89132014000200006 |
| Popis: | The objective of this study was to evaluate the potential of using single tubing-type canister on sperm quality. Semen was collected from the Bali cattle bull by electroejaculation technique and was cryopreserved in liquid nitrogen using slow freezing cryopreservation method. Two type of canister volume was used in this study; commercial canister (342.25π x 278 mm²) and single tubing-type canister (4π x 90 mm²). Makler counting chamber and computer assisted sperm analyzer (CASA) were used to evaluate the sperm motility and viability of post-thaw sperm. Results showed that the bull sperm motility and viability at the bottom of tubing-type canister was statistically higher and significant as compared to the commercial canister (p |
| Databáze: | Directory of Open Access Journals |
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