| Autor: |
Nicolás M. Suárez, Emily Blyth, Kathy Li, Tina Ganzenmueller, Salvatore Camiolo, Selmir Avdic, Barbara Withers, Silvia Linnenweber-Held, Wilfried Gwinner, Akshay Dhingra, Albert Heim, Thomas F. Schulz, Rory Gunson, David Gottlieb, Barry Slobedman, Andrew J. Davison |
| Jazyk: |
angličtina |
| Rok vydání: |
2020 |
| Předmět: |
|
| Zdroj: |
Frontiers in Cellular and Infection Microbiology, Vol 10 (2020) |
| Druh dokumentu: |
article |
| ISSN: |
2235-2988 |
| DOI: |
10.3389/fcimb.2020.00267 |
| Popis: |
Human cytomegalovirus (HCMV) is the most frequent cause of opportunistic viral infection following transplantation. Viral factors of potential clinical importance include the selection of mutants resistant to antiviral drugs and the occurrence of infections involving multiple HCMV strains. These factors are typically addressed by analyzing relevant HCMV genes by PCR and Sanger sequencing, which involves independent assays of limited sensitivity. To assess the dynamics of viral populations with high sensitivity, we applied high-throughput sequencing coupled with HCMV-adapted target enrichment to samples collected longitudinally from 11 transplant recipients (solid organ, n = 9, and allogeneic hematopoietic stem cell, n = 2). Only the latter presented multiple-strain infections. Four cases presented resistance mutations (n = 6), two (A594V and L595S) at high (100%) and four (V715M, V781I, A809V, and T838A) at low ( |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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