| Autor: |
Tejaswini P. Patil, Vishakha S. Parkhe, Somnath S. Kundale, Rajanish K. Kamat, Tukaram D. Dongale, Rajendra S Patil, Arpita P. Tiwari |
| Jazyk: |
angličtina |
| Rok vydání: |
2024 |
| Předmět: |
|
| Zdroj: |
Applied Surface Science Advances, Vol 22, Iss , Pp 100618- (2024) |
| Druh dokumentu: |
article |
| ISSN: |
2666-5239 |
| DOI: |
10.1016/j.apsadv.2024.100618 |
| Popis: |
Nanoconstructs of gold nanoparticles (AuNPs) conjugated with SARS-CoV-2 specific antisense oligonucleotides (ASO) have been utilized to develop sensitive electrochemical nucleic acid biosensor for the detection of SARS-CoV-2 RNA. AuNPs were prepared through a one-pot synthesis method by utilizing Poly-L-Lysine (PLL) biopolymer and as synthesised AuNP were characterized by various analytical techniques such as UV–Vis spectroscopy, X-ray Diffraction (XRD) analysis, Fourier Transform Infra-Red spectroscopy (FT-IR), zeta potential, and Transmission Electron Microscopy (TEM). Poly-L-Lysine functionalized AuNPs (PLL-AuNPs) nanoconstructs platform was employed for immobilization of SARS-CoV-2 specific antisense oligonucleotides (ASO-conjugated PLL-AuNPs) via electrostatic interactions. The PLL-AuNPs were drop casted on glassy carbon electrode (GCE) following immobilization of ASO for fabrication of electrochemical biosensor. The ASO-conjugated PLL-AuNPs nanoconstructs were characterized by cyclic voltammetry (CV), differential pulse voltammetry (DPV), and electrochemical impedance spectroscopy (EIS) techniques. The responsiveness of ASO-conjugated PLL-AuNPs nanoconstructs in presence SARS-CoV-2 RNA was monitored using the DPV, SWV and EIS technique, where methylene blue was employed as an electrochemical indicator for DNA-RNA hybridization detection. The biosensor exhibits a detection range for SARS-CoV-2 RNA infection ranging from 0 to 100 nM, with a limit of detection at 30.2 nM. The electrode, modified with ASO-conjugated PLL-AuNPs, was employed for the detection of SARS-CoV-2 RNA from clinical samples collected from COVID-19-positive individuals. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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