| Autor: |
Ting TAO, Jing YAN, Ziling LIAO, Wenmao ZHANG, Changqiong XU, Ran LI |
| Jazyk: |
angličtina |
| Rok vydání: |
2022 |
| Předmět: |
|
| Zdroj: |
Journal of Holistic Integrative Pharmacy, Vol 3, Iss 4, Pp 380-387 (2022) |
| Druh dokumentu: |
article |
| ISSN: |
2707-3688 |
| DOI: |
10.1016/S2707-3688(23)00006-7 |
| Popis: |
Objective: To investigate pyroptosis in the trophoblast cells HTR-8/Svneo induced by TET2 in oxidized low-density lipoprotein (oxLDL). Methods: HTR-8/Svneo cells were treated with vehicle (0 mg/L) and 50 mg/L oxLDL for 24 h. The effects of oxLDL on TET2 expression in HTR-8/Svneo cells were detected by Western blot. Knockdown of TET2 was performed in HTR-8/Svneo cells using si-TET2. CCK8, Transwell, and wound healing assays were used to detect the effect of TET2 knockdown on the proliferation, cell cycle, migration, and invasion of HTR-8/Svneo cells, respectively. Western blot was used to quantify the expression levels of the pyroptosis proteins GSDMD and caspase-1 in si-TET2 treated cells. Results: oxLDL downregulates TET2 expression in a concentration-dependent manner. TET2 knockdown inhibits HTR-8/Svneo invasion and migration but promotes cell proliferation. Western blot results showed that TET2 knockdown upgregulated the expression of GSDMD and caspase-1. Conclusion: oxLDL induces pyroptosis of HTR-8/Svneo by downregulating TET2 expression, resulting in decreased trophoblast invasion and migration. These data suggest that TET2-induced pyroptosis play a critical role in gestational pathologies, such as preeclampsia. Further studies can clarify the mechanisms of this process and elucidate potential preventive and therapeutic targets. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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