Role of 6-amino flavone in attenuating cisplatin induced neurotoxicity via inhibition of p-JNK signaling pathway in post-natal day-7 mice

Autor: Latafat Kahkashan Ahmad, Rifat Jahan, Amin Jan, Shahid Ali Shah
Jazyk: angličtina
Rok vydání: 2024
Předmět:
Zdroj: Khyber Medical University Journal, Vol 16, Iss 1, Pp 45-51 (2024)
Druh dokumentu: article
ISSN: 2305-2643
2305-2651
DOI: 10.35845/kmuj.2024.23461
Popis: OBJECTIVE: To investigate the protective role of 6-amino flavone (6-AF) in cisplatin-induced neuro-inflammation in the developing brains of post-natal day-7 (PND-7) mice. METHODS: This experimental study, conducted at the Neuro Molecular Medicines Research Center in Peshawar, Pakistan, included 20 PND-7 mice from January to March 2023. PND-7 mice were randomly distributed into four groups, a control group, a cisplatin group, a cisplatin + 6-AF group, and a 6-AF group. Cisplatin was administered intraperitoneally at a dose of 20 mg/kg to the cisplatin group. 6-AF was injected at a dose of 30 mg/kg after cisplatin administration to cisplatin+6-AF group and 6-AF group mice. After 4 hours of the drug treatment, all the PND-7 mice were sacrificed for Western blot analysis. ImageJ software was used for the densitometry of the blots. One-way ANOVA and post-hoc Tukey tests through Prism Graph-5 were applied for statistical analysis. RESULTS: Significant differences in p-JNK levels along with TNF-α, NF-κB, and IL-1β proteins were observed in the brain homogenates of PND-7 mice in various groups. A post-hoc Tukey test revealed a significant increase (p
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