IL-2 and regulation of stress hormones and BDNF neurotropic factor levels after experimental traumatic brain injury (TBI)

Autor: E. E. Fomicheva, S. N. Shanin, T. A. Filatenkova, N. B. Serebryanaya
Jazyk: ruština
Rok vydání: 2020
Předmět:
Zdroj: Медицинская иммунология, Vol 22, Iss 4, Pp 647-656 (2020)
Druh dokumentu: article
ISSN: 1563-0625
2313-741X
DOI: 10.15789/1563-0625-IAR-1973
Popis: Experimental traumatic brain injury (TBI) causes a stable stress response and changes the expression of various cytokine genes and neurotrophic factors. The goal of this study was to reveal changes in the levels of the corticosterone and testosterone hormones and the BDNF cytokine in blood serum, as well as the expression of the BDNF gene in hypothalamus in order to determine the opportunity of correcting the TBI damage with rIL-2. We used a rat model of “dropping load”: mild TBI was caused by falling of the 115 g load from the height of 80 cm, or 120 cm to produce a moderate-degree trauma. After TBI (immediately, or 72 hours later), the rats were injected daily with recombinant human interleukin-2 (Roncoleukin) at a dose of 30 μg/kg, a total of 3 injections. Control animals (also with TBI) received 0.15 M NaCl injections. Blood serum concentrations of corticosterone, testosterone, and BDNF were measured with ELISA tests. BDNF gene expression in hypothalamus was measured using RT-PCR. Results: the experiments showed a relationship between hormone concentrations and severity of head injury. In mild TBI, blood corticosterone levels reached a peak 2 hours after the injury, while in moderate TBI, the peak concentration of corticosterone was lower, being delayed in time (after 24 hours). Corticosterone and testosterone concentrations changed reciprocally in the both groups of injured animals. With injection of rIL-2 in both groups, corticosterone and testosterone levels were significantly increased. On day 7 after TBI, the BDNF level in blood serum was decreased, but it was raised in experimental group that received rIL-2. On day 7, the increase of BDNF gene expression in hypothalamus was more pronounced, when rIL-2 was administered at 72 hours after the head injury. The revealed positive association of BDNF levels and glucocorticoid hormones after mild TBI, like as possible coordination of these parameters with rIL-2 injection after experimental moderate TBI provides a reason to assume that the favorable impact of rIL-2 on the CNS recovery after TBI is, in part, mediated by the mutual modulating interaction of BDNF and glucocorticoid hormones.
Databáze: Directory of Open Access Journals
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