| Autor: |
Mahbub Hasan, Jong-Gu Choi, Sang-Suk Lee |
| Jazyk: |
angličtina |
| Rok vydání: |
2024 |
| Předmět: |
|
| Zdroj: |
AIP Advances, Vol 14, Iss 1, Pp 015128-015128-5 (2024) |
| Druh dokumentu: |
article |
| ISSN: |
2158-3226 |
| DOI: |
10.1063/9.0000638 |
| Popis: |
Drug distribution is a vital parameter for depicting the pharmacokinetic properties of therapeutic agents. Magnetic nanoparticles (MNPs) conjugated with anti-cluster of differentiation 3 (anti-CD3) monoclonal antibody (MNPs-Ab) is a promising immunotherapeutic agent. Magnet-guided targeted delivery of MNPs has been studied exclusively in oncology. The distribution of MNPs is routinely analyzed by measuring Fe levels using inductively coupled mass spectrometry (ICP-MS). However, the ICP-based methods measure the total iron content, including endogenous iron. We used an efficient approach that measured MNPs-Ab distribution after intravenous administration using an in vivo imaging system. The fluorescent-labeled anti-CD3s are conjugated with dextran-coated magnetic nanoparticles (50 nm diameter) by the glutaraldehyde conjugation method. MNPs-Ab was intravenously administered to BALB/c mice. The fluorescence intensity in live animal and organs were analyzed after 0, 1, and 24 h. The conjugation of fluorescently labeled antibodies with MNPs was validated. The attachment of a permanent magnet on the dorsal skin area successfully guided the intravenously injected Ab-MNPs within 24 h. However, after 1 h, no observable fluorescence was seen at the target site. The in vitro organ distribution study confirms nanoparticles’ predominant distribution in the liver and kidneys-the intensities of the fluorescence increase after 24 h than 1 h. Our in vivo imaging studies confirmed the external magnet-driven targeted distribution of the antibody-conjugated nanoparticle. Besides, the in vivo imaging system enables rapidly identifying the distributed nanoparticles. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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