Utilization of RNA sequencing to investigate olfactory dysfunction in chronic rhinosinusitis without nasal polyps: A pilot study

Autor: Jorge A. Gutierrez III, Jeremy L. Barth, Rodney J. Schlosser, Thomas S. Edwards, Timothy L. Smith, Zachary M. Soler
Jazyk: angličtina
Rok vydání: 2024
Předmět:
Zdroj: World Journal of Otorhinolaryngology-Head and Neck Surgery, Vol 10, Iss 1, Pp 29-36 (2024)
Druh dokumentu: article
ISSN: 2589-1081
2095-8811
DOI: 10.1002/wjo2.123
Popis: Abstract Objectives Prior research on olfactory dysfunction in chronic rhinosinusitis (CRS) has focused on patients with polyps and suggests that direct inflammation of the olfactory cleft mucosa plays a contributory role. The purpose of this study was to evaluate gene expression in superior turbinate mucosal specimens, comparing normosmic and dysosmic CRS patients without polyps (CRSsNP). Methods Tissue samples were obtained from the superior turbinates of patients with CRSsNP at the time of endoscopic sinus surgery. Samples subsequently underwent RNA sequencing and functional analysis to investigate biological pathways associated with differentially expressed genes between dysosmic (n = 7) and normosmic (n = 4) patients. Results Differential gene expression analysis comparing dysosmic and normosmic CRSsNP patients showed upregulation of 563 genes and downregulation of 327 genes. Using stringent criteria for multiple comparisons, one upregulated gene (Immediate Early Response 3 [IER3]) had an false discovery rate (FDR) correction adjusted P value considered statistically significant (P
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