| Autor: |
Alpha Kabiné Keita, Aminata Mbaye, Abdoul Karim Soumah, Kadio Jean Jacques Olivier Kadio, Haby Diallo, Thibaut Armel Chérif Gnimadi, Joel Balla Koivogui, Moriba Kowa Povogui, Jean Louis Monemou, Baba Traore, Nicole Vidal, Emilande Guichet, Ahidjo Ayouba, Eric Delaporte, Martine Peeters, Abdoulaye Toure, Alpha Kabinet Keita, AFROSCREEN Team |
| Jazyk: |
angličtina |
| Rok vydání: |
2024 |
| Předmět: |
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| Zdroj: |
Virology Journal, Vol 21, Iss 1, Pp 1-9 (2024) |
| Druh dokumentu: |
article |
| ISSN: |
1743-422X |
| DOI: |
10.1186/s12985-024-02442-7 |
| Popis: |
Abstract Background This study aimed to demonstrate that the genomic material of SARS-CoV-2 can be isolated from strips of COVID-19 rapid diagnostic test cassettes. Method It was a prospective cross-sectional study involving patients admitted to treatment centers and sampling sites in the city of Conakry, Guinea. A total of 121 patients were double sampled, and 9 more patients were tested only for RDT. PCR was conducted according to the protocol of the RunMei kit. Sequencing was performed by using the illumina COVIDSeq protocol. Nine COVID-19 RDTs without nasopharyngeal swabs were in addition tested. Result Among the 130 COVID-19 RDTs, forty-seven were macroscopically positive, whereas seventy-two were positive according to PCR using RDT strip, while among the 121 VTM swabs, sixty-four were positive. Among eighty-three negative COVID-19 RDTs, twenty-seven were positive by PCR using RDT strip with a geometric mean Ct value of 32.49 cycles. Compared to those of PCR using VTM, the sensitivity and specificity of PCR using RDT strip were estimated to be 100% and 85.96%, respectively, with 93.39% test accuracy. Among the fifteen COVID-19 RDT extracts eligible for sequencing, eleven had sequences identical to those obtained via the standard method, with coverage between 75 and 99.6%. Conclusion These results show that COVID-19 RDTs can be used as biological material for the genomic surveillance of SARS-CoV-2. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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