| Autor: |
Rodríguez-Mallon Alina, Cárdenas Yamilet, Lugo Juana, Oliva Aymé, Morales Antonio, Estrada Mario |
| Jazyk: |
angličtina |
| Rok vydání: |
2009 |
| Předmět: |
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| Zdroj: |
Biological Procedures Online, Vol 11, Iss 1, Pp 79-98 (2009) |
| Druh dokumentu: |
article |
| ISSN: |
1480-9222 |
| Popis: |
Abstract Quantization of gene expression requires that an accurate measurement of a specific transcript is made. In this paper, a quantitative reverse transcription-polymerase chain reaction (RT-PCR) by competition for tilapia growth hormone receptor type I is designed and validated. This experimental procedure was used to determine the abundance of growth hormone receptor type I transcript in different tilapia tissues. The results obtained with this developed competitive RT-PCR were similar to real-time PCR results reported recently. This protocol provides a reliable alternative, but less expensive than real-time PCR to quantify specific genes. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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