A Label-Free Aptasensor for Ochratoxin a Detection Based on the Structure Switch of Aptamer

Autor:	Feng Liu, Ailing Ding, Jiushang Zheng, Jiucun Chen, Bin Wang
Jazyk:	angličtina
Rok vydání:	2018
Předmět:	aptamer OTA structure switching ZnPPIX fluorescence Chemical technology TP1-1185
Zdroj:	Sensors, Vol 18, Iss 6, p 1769 (2018)
Druh dokumentu:	article
ISSN:	1424-8220
DOI:	10.3390/s18061769
Popis:	A label-free sensing platform is developed based on switching the structure of aptamer for highly sensitive and selective fluorescence detection of ochratoxin A (OTA). OTA induces the structure of aptamer, transforms into G-quadruplex and produces strong fluorescence in the presence of zinc(II)-protoporphyrin IX probe due to the specific bind to G-quadruplex. The simple method exhibits high sensitivity towards OTA with a detection limit of 0.03 nM and excellent selectivity over other mycotoxins. In addition, the successful detection of OTA in real samples represents a promising application in food safety.
Databáze:	Directory of Open Access Journals
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