Autor: |
Adriana Trapani, Filomena Corbo, Gennaro Agrimi, Nicoletta Ditaranto, Nicola Cioffi, Filippo Perna, Andrea Quivelli, Erika Stefàno, Paola Lunetti, Antonella Muscella, Santo Marsigliante, Antonio Cricenti, Marco Luce, Cristina Mormile, Antonino Cataldo, Stefano Bellucci |
Jazyk: |
angličtina |
Rok vydání: |
2021 |
Předmět: |
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Zdroj: |
Materials, Vol 14, Iss 13, p 3495 (2021) |
Druh dokumentu: |
article |
ISSN: |
1996-1944 |
DOI: |
10.3390/ma14133495 |
Popis: |
Background: The blood–brain barrier (BBB) bypass of dopamine (DA) is still a challenge for supplying it to the neurons of Substantia Nigra mainly affected by Parkinson disease. DA prodrugs have been studied to cross the BBB, overcoming the limitations of DA hydrophilicity. Therefore, the aim of this work is the synthesis and preliminary characterization of an oxidized alginate-dopamine (AlgOX-DA) conjugate conceived for DA nose-to-brain delivery. Methods: A Schiff base was designed to connect oxidized polymeric backbone to DA and both AlgOX and AlgOX-DA were characterized in terms of Raman, XPS, FT-IR, and 1H- NMR spectroscopies, as well as in vitro mucoadhesive and release tests. Results: Data demonstrated that AlgOX-DA was the most mucoadhesive material among the tested ones and it released the neurotransmitter in simulated nasal fluid and in low amounts in phosphate buffer saline. Results also demonstrated the capability of scanning near-field optical microscopy to study the structural and fluorescence properties of AlgOX, fluorescently labeled with fluorescein isothiocyanate microstructures. Interestingly, in SH-SY5Y neuroblastoma cell line up to 100 μg/mL, no toxic effect was derived from AlgOX and AlgOX-DA in 24 h. Conclusions: Overall, the in vitro performances of AlgOX and AlgOX-DA conjugates seem to encourage further ex vivo and in vivo studies in view of nose-to-brain administration. |
Databáze: |
Directory of Open Access Journals |
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