Wedelolactone from Eclipta prostrata (L) L. suppresses inflammation and improves insulin resistance
| Autor: | Trinh Tat Cuong, Duong Duc Thien, Hoang Hai Yen, Nguyen Anh Duc, Trinh Quang Nam, Do Viet Khanh |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2024 |
| Předmět: | |
| Zdroj: | Asian Pacific Journal of Tropical Biomedicine, Vol 14, Iss 6, Pp 253-258 (2024) |
| Druh dokumentu: | article |
| ISSN: | 2221-1691 2588-9222 |
| DOI: | 10.4103/apjtb.apjtb_57_24 |
| Popis: | Objective: To evaluate the effects of wedelolactone, a major flavonoid from Vietnamese Eclipta prostrata (L) L., on inflammation and insulin resistance. Methods: Wedelolactone was extracted from the leaves of Vietnamese Eclipta prostrata (L.) L. with methanol by Soxhlet. The effects of wedelolactone on lipopolysaccharide (LPS)-induced cytokine production, reactive oxygen species (ROS) generation, and nicotinamide adenine dinucleotide phosphate (NADPH) oxidase activities in Raw 264.7 cells were measured by enzyme-linked immunosorbent assay (ELISA), specific immunofluorescent dyes and luminometric analysis, respectively. In addition, its effects on glucose uptake and the protein expression of insulin receptor substrate 1 (IRS1) and glucose transporter 4 (GLUT4) were examined in 3T3-L1 cells by immunofluorescent dyes and Western blot. Results: Wedelolactone at 30 μg/mL significantly inhibited LPS-induced production of tumor necrosis factor-α, interleukin (IL)-6, and IL-8 (P |
| Databáze: | Directory of Open Access Journals |
| Externí odkaz: |
|