microRNA-122 targets the P4HA1 mRNA and regulates its expression in chicken hepatocytes

Autor: Xingguo Wang, Jianfeng Yu, Fang Shao, Yanping Zhang, Yanyan Li, Xiangyun Lu, Daoqing Gong, Zhiliang Gu
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Zdroj: Italian Journal of Animal Science, Vol 18, Iss 1, Pp 587-593 (2019)
Druh dokumentu: article
ISSN: 1828-051X
1828051X
DOI: 10.1080/1828051X.2018.1548912
Popis: microRNAs (miRNAs) are small non-coding RNAs that regulate various biological processes by targeting mRNAs and affecting their stability or translation. miR-122 is a major miRNA in mammalian liver but its function in poultry liver has not been studied. Therefore, the objective of the current study was to determine the function of miR-122 in the chicken liver. Through bioinformatics, we found P4HA1 was a candidate target of miR-122. P4HA1 mRNA and protein expression were increased in hepatocytes when miR-122 was knocked down by locked nucleic acid (LNA)-antisense-miR-122 (LNA-122). Reporter gene analyses confirmed that P4HA1 was a target of miR-122. Because P4HA1 is known to play an important role in liver metabolism, these findings suggest that miR-122 may be involved in liver metabolism in the chicken by regulating the P4HA1 expression.Highlights The predicted target site of miR-122 in chicken P4HA1 is conserved among species. P4HA1 mRNA and protein are upregulated in miR-122 knockdown chicken hepatocytes. miR-122 targets P4HA1 in chicken.
Databáze: Directory of Open Access Journals
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