Comparative characteristic of lung cancer stem-like cells generated in vitro under different culture conditions
| Autor: | O. V. Skachkova, O. I. Gorbach, M. V. Inomistova, L. V. Garmanchuk, N. M. Khranovska |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: | |
| Zdroj: | The Ukrainian Biochemical Journal, Vol 93, Iss 1, Pp 88-95 (2021) |
| Druh dokumentu: | article |
| ISSN: | 2409-4943 2413-5003 |
| DOI: | 10.15407/ubj93.01.088 |
| Popis: | Cancer stem cells (CSCs) play an important role in resistance to cancer treatment and recurrence developing. The aim of this study was to obtain cell culture of MOR line non-small cell lung cancer cells enriched in CSCs and to investigate its functional and molecular-genetic properties. Tumor spheroids (TS) of MOR cell line were generated in vitro under normal adhesive (0.2% carboxymethyl cellulose, CMC) or low-adhesive (2% agarose) culture conditions. Lateral population of TS was evaluated by flow cytometry with the use of R-123 fluorescent dye, the index of R-123 exclusion was also assessed. Expression of CD44, ALDHA1, CD133, Sox2 and Nanog mRNA was determined with RT-qPCR. It was found that regardless of the culture conditions tumor spheroids form a lateral population characterized by an increased dye exclusion index. Expression levels of CD44, ALDHA1, CD133, Sox2 and Nanog mRNA in TS cells obtained under low-adhesive (2% agarose) conditions were significantly higher than in monolayer cells and cells obtained using 0.2% CMC. Thus, the proposed method of culturing in low-adhesive conditions allowed to enrich significantly tumor spheroids of MOR line in cells with CSC properties. |
| Databáze: | Directory of Open Access Journals |
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