Repeated centrifuging and washing concentrates bacterial samples in peritoneal dialysis for optimal culture: an original article

Autor: Ni Tien, Bang-Jau You, Hsuan-Jen Lin, Chieh-Ying Chang, Che-Yi Chou, Hsiu-Shen Lin, Chiz-Tzung Chang, Charles C. N. Wang, Hung-Chih Chen
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Zdroj: BMC Microbiology, Vol 20, Iss 1, Pp 1-9 (2020)
Druh dokumentu: article
ISSN: 1471-2180
DOI: 10.1186/s12866-020-02044-7
Popis: Abstract Background Bacterial cultures allow the identification of infectious disease pathogens. However, obtaining the results of conventional culture methods is time-consuming, taking at least two days. A more efficient alternative is the use of concentrated bacterial samples to accelerate culture growth. Our study focuses on the development of a high-yield sample concentrating technique. Results A total of 71 paired samples were obtained from patients on peritoneal dialysis (PD). The peritoneal dialysates were repeat-centrifuged and then washed with saline, namely the centrifuging and washing method (C&W method). The concentrated samples were Gram-stained and inoculated into culture plates. The equivalent unprocessed dialysates were cultured as the reference method. The times until culture results for the two methods were compared. The reference method yielded no positive Gram stain results, but the C&W method immediately gave positive Gram stain results for 28 samples (p
Databáze: Directory of Open Access Journals
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