| Autor: |
Maria Tizu, Ion Mărunțelu, Bogdan Mihai Cristea, Claudiu Nistor, Nikolay Ishkitiev, Zornitsa Mihaylova, Rozaliya Tsikandelova, Marina Miteva, Ana Caruntu, Cristina Sabliov, Bogdan Calenic, Ileana Constantinescu |
| Jazyk: |
angličtina |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
Journal of Functional Biomaterials, Vol 13, Iss 3, p 109 (2022) |
| Druh dokumentu: |
article |
| ISSN: |
2079-4983 |
| DOI: |
10.3390/jfb13030109 |
| Popis: |
Polymeric nanoparticles have been introduced as a delivery vehicle for active compounds in a broad range of medical applications due to their biocompatibility, stability, controlled release of active compounds, and reduced toxicity. The oral route is the most used approach for delivery of biologics to the body. The homeostasis and function of oral cavity tissues are dependent on the activity of stem cells. The present work focuses, for the first time, on the interaction between two types of polymeric nanoparticles, poly (lactic-co-glycolic acid) or PLGA and PLGA/chitosan, and two stem cell populations, oral keratinocyte stem cells (OKSCs) and stem cells from human exfoliated deciduous teeth (SHEDs). The main results show that statistical significance was observed in OKSCs uptake when compared with normal keratinocytes and transit amplifying cells after 24 h of incubation with 5 and 10 µg/mL PLGA/chitosan. The CD117+ SHED subpopulation incorporated more PLGA/chitosan nanoparticles than nonseparated SHED. The uptake for PLGA/chitosan particles was better than for PLGA particles with longer incubation times, yielding better results in both cell types. The present results demonstrate that nanoparticle uptake depends on stem cell type, incubation time, particle concentration, and surface properties. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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