Molecular Characterization of the Recombinant Ig1 Axl Receptor Domain: An Intriguing Bait for Screening in Drug Discovery

Autor:	Rossella Di Stasi, Lucia De Rosa, Guido Izzi, Luca Domenico D’Andrea
Jazyk:	angličtina
Rok vydání:	2024
Předmět:	Axl receptor recombinant Ig1 domain protein refolding drug discovery circular dichroism fluorescence spectroscopy Organic chemistry QD241-441
Zdroj:	Molecules, Vol 29, Iss 2, p 521 (2024)
Druh dokumentu:	article
ISSN:	1420-3049 91396573
DOI:	10.3390/molecules29020521
Popis:	Axl receptor tyrosine kinase and its ligand Gas6 regulate several biological processes and are involved in both the onset and progression of tumor malignancies and autoimmune diseases. Based on its key role in these settings, Axl is considered a promising target for the development of molecules with therapeutic and diagnostic purposes. In this paper, we describe the molecular characterization of the recombinant Ig1 domain of Axl (Ig1 Axl) and its biochemical properties. For the first time, an exhaustive spectroscopic characterization of the recombinant protein through circular dichroism and fluorescence studies is also reported, as well as a binding analysis to its natural ligand Gas6, paving the way for the use of recombinant Ig1 Axl as a bait in drug discovery screening procedures aimed at the identification of novel and specific binders targeting the Axl receptor.
Databáze:	Directory of Open Access Journals
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