Exploring the Therapeutic Potential of Bee Venom Components in Wound Healing: A Comprehensive Evaluation of Morphometric, Biochemical, and Histopathological Markers

Autor: Denk Bariş, Yaprakci Volkan, Dayi Belma, Sevimli Alper, Stevanović Jevrosima, Glavinić Uroš, Stanimirović Zoran
Jazyk: angličtina
Rok vydání: 2024
Předmět:
Zdroj: Acta Veterinaria, Vol 74, Iss 3, Pp 347-366 (2024)
Druh dokumentu: article
ISSN: 1820-7448
DOI: 10.2478/acve-2024-0025
Popis: Bee venom (BV) and its components, secretory phospholipase A2 (sPLA2) and Apis cerana secapin-1 (AcSecapin-1), have potential effects on wound healing. This study aims to evaluate impact of BV, sPLA2, and AcSecapin-1 on full-thickness wound healing in male Wistar Albino rats over a 7-day period. Various morphometric (body weight, wound contraction), biochemical (hydroxyproline, oleic acid, IL-8, TGF-β1, redox parameters), and histopathological markers (reepithelialization, inflammatory cells, angiogenesis, fibroblast activation, and collagen density) were assessed. Treatment with sPLA2 and AcSecapin-1 increased oleic acid levels. IL-8 levels increased with sPLA2 treatment, and TGF-β1 levels increased with AcSecapin-1 treatment. BV and its components led to a decrease in FRAP levels. Additionally, BV treatment resulted in reduced angiogenesis, and both BV and sPLA2 treatments reduced inflammatory cells. All groups exhibited wound contraction without delay or regression. sPLA2 and AcSecapin-1 induced alterations in the wound healing milieu, without systemic changes. The treatment groups, except for the AcSecapin-1 group, showed an anti-inflammatory effect, identified by reduced inflammatory cell accumulation. Only the BV treatment suppressed angiogenesis. In conclusion, BV, sPLA2, and AcSecapin-1 demonstrate distinct effects on wound healing, with BV showing notable anti-inflammatory and anti-angiogenic properties, while sPLA2 and AcSecapin-1 influenced cytokine and oleic acid levels.
Databáze: Directory of Open Access Journals