Simple Protocol for immunoglobulin G Purification from Camel 'Camelus dromedarius' Serum
| Autor: | Shawki Asmaa, Abd El-Baky Nawal, Ahmed Mohammed, Linjawi Mustafa H., Aljaddawi Abdullah A., Redwan Elrashdy M. |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: | |
| Zdroj: | Open Life Sciences, Vol 12, Iss 1, Pp 143-155 (2017) |
| Druh dokumentu: | article |
| ISSN: | 2391-5412 2017-0017 |
| DOI: | 10.1515/biol-2017-0017 |
| Popis: | The present study aimed to describe and standardize a simple and efficient protocol for purification of camel IgG from serum, which can be applied for Camilidae antibody production in research laboratories, the preindustrial stage. Camel serum IgG was separated with caprylic acid and ammonium sulfate, then the effect of four variables studied: caprylic acid concentration, pH, stirring time, and stirring intensity. Camel IgG prepared by standardized caprylic acid fractionation method for camel serum was compared with commercial anti-sera products. Camel IgG purification from undiluted sera using caprylic acid at concentration of 8% v/v gave the best results. Purification at different pH values using caprylic acid at 8% v/v revealed that pH 5.5 was optimal. Investigating purification at different stirring time intervals using 8% v/v caprylic acid at pH 5.5 demonstrated that stirring for 90 min gave the optimum results. Finally, studying purification at different stirring intensities using 8% v/v caprylic acid at pH 5.5 for 90 min, the best stirring intensity was at 450 rpm. Overall, the results suggest that caprylic acid purification of camel serum IgG is more effective and safe than ammonium sulfate method in simplicity, purity, and lower non-IgG proteins in the final preparation with lower protein aggregates. |
| Databáze: | Directory of Open Access Journals |
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