Mutant alleles associated to chloroquine and sulfadoxine-pyrimethanime resistance in Plasmodium falciparum of the Ecuador-Peru and Ecuador-Colombia borders
| Autor: | Nancy Arróspide, Gisely Hijar-Guerra, Doménica de Mora, César Eduardo Diaz-Cortéz, Raúl Veloz-Perez, Sonia Gutierrez, César Cabezas _Sánchez |
|---|---|
| Jazyk: | Spanish; Castilian |
| Rok vydání: | 2014 |
| Předmět: | |
| Zdroj: | Revista Peruana de Medicina Experimental y Salud Pública, Vol 31, Iss 2 (2014) |
| Druh dokumentu: | article |
| ISSN: | 1726-4634 1726-4642 |
| DOI: | 10.17843/rpmesp.2014.312.47 |
| Popis: | The frequency of mutations in pfCRT and DHFR/DHPS genes of Plasmodium falciparum associated with resistance to chloroquine and sulfadoxine-pyrimethamine was evaluated in 83 strains from the districts of Esmeralda and Machala, located on the borders of Ecuador-Peru and Ecuador-Colombia in 2002. Polymerase chain reaction (PCR), conventional and its variants, was used. Mutations in the pfCRT gene were found in more than 90% of the samples from Esmeralda and Machala. For the DHFR gene, 90% of the strains were mutant samples from Esmeralda, 3 were double mutations and 1 was a triple mutation. In Machala, 25% were simple mutant forms and 75% mixed mutant forms (wild forms/mutant). In conclusion, resistance to chloroquine has been fixed in strains carrying K76T pfCRT mutation, whereas genetic imprinting for resistance to pyrimethamine is evolving, particularly in the district of Esmeralda. |
| Databáze: | Directory of Open Access Journals |
| Externí odkaz: |
|