The Role of Withania somnifera (Ashwagandha) and Omega-3 Fatty Acids on TNF-α and Joint Inflammation in an Animal Model of Rheumatoid Arthritis
| Autor: | Shweta Singh, Rajendra Nath, Rishi Pal, Anju Mehrotra, Promod Kumar Singh, Rakesh Kumar Dixit, Sarvesh Singh, Rahul Kumar |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: | |
| Zdroj: | Journal of Clinical and Diagnostic Research, Vol 13, Iss 4, Pp FF01-FF05 (2019) |
| Druh dokumentu: | article |
| ISSN: | 2249-782X 0973-709X |
| DOI: | 10.7860/JCDR/2019/40947.12787 |
| Popis: | Introduction: Rheumatoid Arthritis (RA) is an autoimmune disorder characterised by progressive joint destruction leading to severe disability. The existing management of RA includes Nonsteroidal Anti-Inflammatory Drugs (NSAIDs), DiseaseModifying Antirheumatic Drugs (DMARDs) and biological such as Tumour Necrosis Factor (TNF) inhibitors but search for alternative and safer therapy is still going on. Aim: To evaluate the efficacy of the combination of Withania somnifera (WS) and omega-3 fatty acids on TNF alpha level and joint histopathology in the treatment of Complete Freund’s Adjuvant (CFA) induced rheumatoid arthritis in a rat model. Materials and Methods: Healthy adult male Wistar albino rats were divided into six groups containing six rats in each group (n=36). Group I served as arthritis control, Group II and III received WS in dose of 500 mg/kg and 1000 mg/kg respectively. Group IV received Omega-3 polyunsaturated fatty acids in dose of 100 mg/kg. The combination of WS (1000 mg/kg) and Omega-3 polyunsaturated fatty acids (100 mg/kg) was given to rats of group V. Group VI served as standard treatment group and received Indomethacin 3 mg/kg. Arthritis was induced in groups II to VI by CFA. TNF-α was determined on day 0, 10 and 21. On day 21 all rats were sacrificed and inflamed limbs were excised above the ankle joints and examined for a pathological finding of RA. The data was analysed by one-way analysis of variance (ANOVA) followed by Dunnett’s test and Tukey test. The Dunnett’s test compares each mean to a control mean and simultaneous comparison between all other pairs was done by Tukey test. Results: WS in both doses showed significant reduction in TNF-α (p |
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