Puromycin-induced kidney injury and subsequent regeneration in adult zebrafish

Autor: Soonil Koun, Hye-jin Park, Su-min Jung, Jin Joo Cha, Dae Ryong Cha, Young Sun Kang
Jazyk: angličtina
Rok vydání: 2023
Předmět:
Zdroj: Animal Cells and Systems, Vol 27, Iss 1, Pp 112-119 (2023)
Druh dokumentu: article
ISSN: 19768354
2151-2485
1976-8354
DOI: 10.1080/19768354.2023.2203211
Popis: ABSTRACTPuromycin treatment can cause glomerular injury to the kidney, leading to proteinuria. However, the pathogenesis of acute kidney injury and subsequent regeneration after puromycin administration in animal models remain unclear. In this work, we examined the characteristics of kidney injury and subsequent regeneration following puromycin treatment in adult zebrafish. We intraperitoneally injected 100 μg of puromycin into zebrafish; sacrificed them at 1, 3, 5, 7, or 14 days post-injection (dpi); and examined the morphological, functional, and molecular changes in the kidney. Puromycin-treated zebrafish presented more rapid clearance of rhodamine dextran than control animals. Morphological changes were observed immediately after the puromycin injection (1–7 dpi) and had recovered by 14 dpi. The mRNA production of lhx1a, a renal progenitor marker, increased during recovery from kidney injury. Levels of NFκB, TNFα, Nampt, and p-ERK increased significantly during nephron injury and regeneration, and Sirt1, FOXO1, pax2, and wt1b showed an increasing tendency. However, TGF-β1 and smad5 production did not show any changes after puromycin treatment. This study provides evidence that puromycin-induced injury in adult zebrafish kidneys is a potential tool for evaluating the mechanism of nephron injury and subsequent regeneration.
Databáze: Directory of Open Access Journals
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