Insertion of a Suicide Gene into an Immortalized Human Hepatocyte Cell Line
| Autor: | Naoya Kobayashi M.D., Ph.D., Hirofumi Noguchi, Toshinori Totsugawa, Takamasa Watanabe, Toshihisa Matsumura, Toshiyoshi Fujiwara, Masahiro Miyazaki, Kenichi Fukaya, Masayoshi Namba, Noriaki Tanaka |
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| Jazyk: | angličtina |
| Rok vydání: | 2001 |
| Předmět: | |
| Zdroj: | Cell Transplantation, Vol 10 (2001) |
| Druh dokumentu: | article |
| ISSN: | 0963-6897 1555-3892 00000000 |
| DOI: | 10.3727/000000001783986648 |
| Popis: | For developing a bioartificial liver (BAL) device, an attractive alternative to the primary human hepatocytes would be the use of highly differentiated immortalized human hepatocytes with a safeguard. To test the feasibility, the primary human hepatocytes were immortalized by a plasmid SV3neo encoding simian virus 40 large T antigen (SV40Tag) gene. A highly differentiated hepatocyte line OUMS-29 was established. A suicide gene of herpes simplex virus-thymidine kinase (HSV-TK) was retrovirally introduced into OUMS-29 cells as a safeguard for clinical application. One of the resulting HSV-TK-positive cell lines, OUMS-29/ tk, grew in chemically defined serum-free medium with the gene expression of differentiated liver functions. OUMS-29/tk cells were 100 times more sensitive to ganciclovir compared with unmodified OUMS-29 cells in in vitro experiments. We have established a tightly regulated immortalized human hepatocyte cell line. Essentially unlimited availability of OUMS-29/tk cells may be clinically useful for BAL therapy. |
| Databáze: | Directory of Open Access Journals |
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