Sustainable Synthesis of Biopolymer Polyhydroxybutyrate (PHB) from Agro-residue by Brevibacterium casei with Emphasis on Degradation Analysis
| Autor: | R. Preethi Rathna, M. Kulandhaivel |
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| Jazyk: | angličtina |
| Rok vydání: | 2024 |
| Předmět: | |
| Zdroj: | Journal of Pure and Applied Microbiology, Vol 18, Iss 1, Pp 347-366 (2024) |
| Druh dokumentu: | article |
| ISSN: | 0973-7510 2581-690X |
| DOI: | 10.22207/JPAM.18.1.18 |
| Popis: | The Polyhydroxybutyrate (PHB) polymer is a biodegradable microbial polyester that is intracellularly accruing due to the depletion of nitrogen and phosphorous resources and an increase in carbon supply. As part of this research investigation, Sudan Black B staining, fermentation, chloroform-sodium hypochlorite solvent-based extraction, and characterization of extracted PHB were used to isolate and identify organisms capable of producing PHB. Brevibacterium casei (OQ519751) was used to synthesize PHB biopolymer from agro-residues (orange peel, mangosteen peel, sugarcane bagasse, water hyacinth, and jackfruit peel). Using the Response Surface Methodology (RSM) and the Central Composite Design (CCD) has proven to be highly effective for optimizing PHB synthesis. The optimal conditions determined through RSM allowed Brevibacterium casei to produce significant amounts of PHB when compared to an unoptimized medium. The model demonstrated statistical significance, as indicated by the F-value of 19.96 with an associated p-value of |
| Databáze: | Directory of Open Access Journals |
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