| Autor: |
Christian Dullin, Jonas Albers, Aishwarya Tagat, Andrea Lorenzon, Lorenzo D'Amico, Sabina Chiriotti, Nicola Sodini, Diego Dreossi, Frauke Alves, Anna Bergamaschi, Giuliana Tromba |
| Jazyk: |
angličtina |
| Rok vydání: |
2024 |
| Předmět: |
|
| Zdroj: |
Frontiers in Medicine, Vol 11 (2024) |
| Druh dokumentu: |
article |
| ISSN: |
2296-858X |
| DOI: |
10.3389/fmed.2024.1338846 |
| Popis: |
IntroductionSynchrotron-based propagation-based imaging (PBI) is ideally suited for lung imaging and has successfully been applied in a variety of in vivo small animal studies. Virtually all these experiments were tailored to achieve extremely high spatial resolution close to the alveolar level while delivering high x-ray doses that would not permit longitudinal studies. However, the main rationale for performing lung imaging studies in vivo in small animal models is the ability to follow disease progression or monitor treatment response in the same animal over time. Thus, an in vivo imaging strategy should ideally allow performing longitudinal studies.MethodsHere, we demonstrate our findings of using PBI-based planar and CT imaging with two different detectors—MÖNCH 0.3 direct conversion detector and a complementary metal-oxide-semiconductor (CMOS) detector (Photonics Science)—in an Ovalbumin induced experimental allergic airway disease mouse model in comparison with healthy controls. The mice were imaged free breathing under isoflurane anesthesia.ResultsAt x-ray dose levels below those once used by commercial small animal CT devices at similar spatial resolutions, we were able to resolve structural changes at a pixel size down to 25 μm and demonstrate the reduction in elastic recoil in the asthmatic mice in cinematic planar x-ray imaging with a frame rate of up to 100 fps.DiscussionThus, we believe that our approach will permit longitudinal small animal lung disease studies, closely following the mice over longer time spans. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
|
