| Autor: |
ZHOU Hua-teng, LI Chang-ming, WANG Li-xiang, HU Jin-tao, DU Wei-bin, ZHANG He-lou, REN Wei-fan |
| Jazyk: |
čínština |
| Rok vydání: |
2020 |
| Předmět: |
|
| Zdroj: |
Jichu yixue yu linchuang, Vol 40, Iss 11, Pp 1468-1473 (2020) |
| Druh dokumentu: |
article |
| ISSN: |
1001-6325 |
| Popis: |
Objective To differentiate urine derived pluripotent stem cells (iPSCs) into hair follicle stem cells (HFSCs) to construct a skin substitutes. Methods Human urinary cells isolated from a healthy donor were re-programmed into iPSCs. iPSCs were then induced into iPS-HFSCs. Immunocytochemistry and RT-qPCR were used to examine the characteristics of the induced iPS-HFSCs. Results The iPSCs induced from urine cells have characteristics as embryonic stem cells. iPSCs derived cells displayed the morphology, genes expression and phenotypes similar to iPS-HFSCs. Conclusions The urine cells are successfully reprogramed into iPSCs by four transcription factors, and iPSCs derived cells show characteristics similar to HFSCs. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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