Nitric Oxide-Induced Apoptosis in Human Granulosa Cells
| Autor: | Ali Reza Zamani, Iraj Amiri, Ebrahim Nasiri, Mohsen Pourghasem |
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| Jazyk: | angličtina |
| Rok vydání: | 2005 |
| Předmět: | |
| Zdroj: | Cell Journal, Vol 7, Iss 2, Pp 80-85 (2005) |
| Druh dokumentu: | article |
| ISSN: | 2228-5806 2228-5814 |
| Popis: | Introduction: Recent evidence suggests that nitric oxide (NO) acts as an important factor in a variety of physiological and pathological processes, including reproductive function. The purpose of the present study was to investigate whether NO might significantly induce any apoptotic changes in cultured human granulosa cells. Material and Methods: The granulosa cells (GC) were obtained from women taking part in an in vitro fertilization (IVF) program. After 48h culture, 1mM DETA/NO was added to the culture medium and then the apoptosis of granulosa cells was evaluated by in situ terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) immediately and after one hour culture. Results: Nitric oxide significantly increased apoptotic index after one hour in human granulosa cell culture (p |
| Databáze: | Directory of Open Access Journals |
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