Cloning, purification and characterization of human dentine matrix protein 1(DMP1)

Autor: Tarek El-Bialy, Dror Aizenbud, Hamid Sadeghi, Paul Scott, Itay Aizenbud
Jazyk: angličtina
Rok vydání: 2023
Předmět:
Zdroj: Saudi Dental Journal, Vol 35, Iss 5, Pp 553-558 (2023)
Druh dokumentu: article
ISSN: 1013-9052
DOI: 10.1016/j.sdentj.2023.05.006
Popis: Introduction: Human teeth are composed mainly of dentin, formed by the odontoblasts. Dentin matrix protein 1 (DMP1) is one of odontoblast differentiation's most important growth factors. Human DMP1 has yet to be completely identified or studied. This study aimed to clone and characterize human DMP1. Materials and methods: The DMP1 gene sequence was prepared and cloned by transfection of human 293 cells. Results: The recombinant DMP1 was purified and characterized. Conclusion: The results suggested its future use in dental tissue regeneration and tissue engineering.
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