In vivo spatiotemporal characterizing diverse body transportation of optical labeled high immunity aluminium adjuvants with photoacoustic tomography
| Autor: | Fan Meng, Chaohao Liang, Barkat Ali, Changwu Wan, Fengbing He, Jiarui Chen, Yiqing Zhang, Zhijia Luo, Lingling Su, Xiaoya Zhao, Bin Yang, Jian Zhang |
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| Jazyk: | angličtina |
| Rok vydání: | 2024 |
| Předmět: | |
| Zdroj: | Photoacoustics, Vol 39, Iss , Pp 100643- (2024) |
| Druh dokumentu: | article |
| ISSN: | 2213-5979 78677238 |
| DOI: | 10.1016/j.pacs.2024.100643 |
| Popis: | Vaccine development requires high-resolution, in situ, and visual adjuvant technology. To address this need, this work proposed a novel adjuvant labeling that involved indocyanine green (ICG) and bovine serum albumin (BSA) with self-assembled aluminium adjuvant (Alum), which was called BSA@ICG@Alum. This compound exhibited excellent photoacoustic properties and has been confirmed its safety, biocompatibility, high antigen binding efficiency, and superior induction of immune response. Photoacoustic tomography (PAT) tracked the distribution of Alum in lymph nodes (LNs) and lymphatic vessels in real time after diverse injection modalities. The non-invasive imaging approach revealed that BSA@ICG@Alum was transported to the draining LNs 60 min after intramuscular injection and to distal LNs within 30 min after lymph node injection. In conclusion, PAT enabled real-time three-dimensional and quantitative visualization, thus offering a powerful tool for advancing vaccine design by providing critical insights into adjuvant transport and immune system activation. |
| Databáze: | Directory of Open Access Journals |
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