| Autor: |
Widayanti Tika, Pambudi Sabar, Irawan Setyo Utomo Doddy, Maryam Romsyah, Suryanggono Jodi, Nurlaila Ika, Sulfianti Asri |
| Jazyk: |
English<br />French |
| Rok vydání: |
2024 |
| Předmět: |
|
| Zdroj: |
BIO Web of Conferences, Vol 127, p 04003 (2024) |
| Druh dokumentu: |
article |
| ISSN: |
2117-4458 |
| DOI: |
10.1051/bioconf/202412704003 |
| Popis: |
Foot-and-mouth disease (FMD) is a highly contagious disease that infects cloven-hoofed animals, becoming a serious threat to livestock production and leading to significant economic losses. The re-occurring FMD outbreak in Indonesia was reported back in 2022, causing hundreds of cattle deaths. The immunogenic viral capsid VP1 protein has been extensively researched as a vaccine candidate despite the fact that the existing FMD vaccine uses an inactivated virus. The vp1 gene (648 bps) from FMD virus serotype O was integrated into pET-32b vector and transformed into Escherichia coli TOP10F’. The recombinant pET32b-VP1-1D plasmid was expressed in E. coli BL21(DE3), followed by N-terminal His tag purification. Protein profiles were determined with SDS-PAGE, showing the target protein at 33KDa. Five 6-week-old BALB/c mice were administered intraperitoneal injections of 50 μg and 100 μg protein, respectively, with two booster shots within two-week intervals. The immune response of polyclonal antibodies was tested using indirect ELISA, resulting in a high absorbance signal compared to non-immunized mice. Thus, the outcomes demonstrate that the VP1 recombinant protein from this study has potential as an immunogen in FMD vaccine development. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
|
