Detection and quantification of infectious severe acute respiratory coronavirus-2 in diverse clinical and environmental samples

Autor: Yi-Chan Lin, Rebecca J. Malott, Linda Ward, Linet Kiplagat, Kanti Pabbaraju, Kara Gill, Byron M. Berenger, Jia Hu, Kevin Fonseca, Ryan S. Noyce, Thomas Louie, David H. Evans, John M. Conly
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Zdroj: Scientific Reports, Vol 12, Iss 1, Pp 1-19 (2022)
Druh dokumentu: article
ISSN: 2045-2322
76415074
DOI: 10.1038/s41598-022-09218-5
Popis: Abstract To explore the potential modes of Severe Acute Respiratory Coronavirus-2 (SARS-CoV-2) transmission, we collected 535 diverse clinical and environmental samples from 75 infected hospitalized and community patients. Infectious SARS-CoV-2 with quantitative burdens varying from 5 plaque-forming units/mL (PFU/mL) up to 1.0 × 106 PFU/mL was detected in 151/459 (33%) of the specimens assayed and up to 1.3 × 106 PFU/mL on fomites with confirmation by plaque morphology, PCR, immunohistochemistry, and/or sequencing. Infectious virus in clinical and associated environmental samples correlated with time since symptom onset with no detection after 7–8 days in immunocompetent hosts and with N-gene based Ct values ≤ 25 significantly predictive of yielding plaques in culture. SARS-CoV-2 isolated from patient respiratory tract samples caused illness in a hamster model with a minimum infectious dose of ≤ 14 PFU. Together, our findings offer compelling evidence that large respiratory droplet and contact (direct and indirect i.e., fomites) are important modes of SARS-CoV-2 transmission.
Databáze: Directory of Open Access Journals
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