| Autor: |
Ratka Hoferick, Holger Schönherr, Stéphan Barbe |
| Jazyk: |
angličtina |
| Rok vydání: |
2023 |
| Předmět: |
|
| Zdroj: |
Fluids, Vol 9, Iss 1, p 10 (2023) |
| Druh dokumentu: |
article |
| ISSN: |
2311-5521 |
| DOI: |
10.3390/fluids9010010 |
| Popis: |
This research explores the two-phase flow behavior involved in enhanced dense phase carbon dioxide inactivation of E. coli DH5α, which has been shown to possess a high microbial reduction efficiency of up to 3.7 ± 0.4 log. We present an experiment in which the liquid sample was pressurized with liquid carbon dioxide to 8.2 MPa and, after saturation, was forced to flow through a mini tube. An experimental setup was developed to visualize the flow patterns (plug, slug and churn flows) occurring in the mini tube by means of high-speed imaging. The values of the wall shear stress were estimated within the mini tube with the help of the gas slug velocities (8–9 m/s) and were compared with threshold shear stress values reported for the disruption of fresh E. coli cells. The results suggest that the preliminary pressurization phase may cause a substantial destabilization of the cell wall of E. coli DH5α. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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