Regulatory roles of long non-coding RNAs in short-term heat stress in adult worker bees

Autor: Bing Zhang, Chaoying Zhang, Jiangchao Zhang, Surong Lu, Huiting Zhao, Yusuo Jiang, Weihua Ma
Jazyk: angličtina
Rok vydání: 2024
Předmět:
Zdroj: BMC Genomics, Vol 25, Iss 1, Pp 1-13 (2024)
Druh dokumentu: article
ISSN: 1471-2164
DOI: 10.1186/s12864-024-10399-8
Popis: Abstract Long non-coding RNAs (lncRNAs) are crucial modulators of post-transcriptional gene expression regulation, cell fate determination, and disease development. However, lncRNA functions during short-term heat stress in adult worker bees are poorly understood. Here, we performed deep sequencing and bioinformatic analyses of honeybee lncRNAs. RNA interference was performed by using siRNA targeting the most highly expressed lncRNA. The silencing effect on lncRNA and the relative expression levels of seven heat shock protein (HSP) genes, were subsequently examined. Overall, 7,842 lncRNAs and 115 differentially expressed lncRNAs (DELs) were identified in adult worker bees following heat stress exposure. Structural analysis revealed that the overall expression abundance, length of transcripts, exon number, and open reading frames of lncRNAs were lower than those of mRNAs. GO analysis revealed that the target genes were mainly involved in “metabolism,” “protein folding,” “response to stress,” and “signal transduction” pathways. KEGG analysis indicated that the “protein processing in endoplasmic reticulum” and “longevity regulating pathway-multiple species” pathways were most enriched. Quantitative real-time polymerase chain reaction (qRT-PCR) detection of the selected DELs confirmed the reliability of the sequencing data. Moreover, the siRNA experiment indicated that feeding siRNA yielded a silencing efficiency of 77.51% for lncRNA MSTRG.9645.5. Upon silencing this lncRNA, the expression levels of three HSP genes were significantly downregulated (p
Databáze: Directory of Open Access Journals
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