A simple, quantitative assay for the detection of viable but non-culturable (VBNC) bacteria

Autor: Ignacio Servando Arvizu, Sean Richard Murray
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Zdroj: STAR Protocols, Vol 2, Iss 3, Pp 100738- (2021)
Druh dokumentu: article
ISSN: 2666-1667
DOI: 10.1016/j.xpro.2021.100738
Popis: Summary: This protocol determines the fraction of a bacterial population that is viable and culturable, viable and non-culturable, or non-viable (dead). Each population is detected by isolating colonies on agar plates, performing direct counts, and staining for live or dead cells. Its application is limited to bacteria that are stainable and when permissible growth conditions are known. The quantitative data extracted allow for the detection of a viable but non-culturable (alive and non-dividing) population from a liquid culture.For complete details on the use and execution of this protocol, please refer to Stott et al. (2015).
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