Antibacterial activity of a Tribolium castaneum defensin in an in vitro infection model of Streptococcus pneumoniae
| Autor: | Nora S. Lindhauer, Wilhelm Bertrams, Anne Pöppel, Christina E. Herkt, Andre Wesener, Kerstin Hoffmann, Brandon Greene, Mark Van Der Linden, Andreas Vilcinskas, Kerstin Seidel, Bernd Schmeck |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: | |
| Zdroj: | Virulence, Vol 10, Iss 1, Pp 902-909 (2019) |
| Druh dokumentu: | article |
| ISSN: | 2150-5594 2150-5608 21505594 |
| DOI: | 10.1080/21505594.2019.1685150 |
| Popis: | Streptococcus pneumoniae (S. pneumoniae) is the most common bacterial cause of community-acquired pneumonia. Increasing rates of antibiotic-resistant S. pneumoniae strains impair therapy and necessitate alternative treatment options. In this study, we analysed insect-derived antimicrobial peptides (AMPs) for antibacterial effects on S. pneumoniae in a human in vitro infection model. AMP effects on bacterial growth were examined by colony forming unit (CFU)-assays, and growth curve measurements. Furthermore, cytotoxicity to primary human macrophages was detected by measuring lactate-dehydrogenase release to the supernatant. One AMP (Defensin 1) was tested in a model of primary human monocyte-derived macrophages infected with S. pneumoniae strain D39 and a multi-resistant clinical isolate. Inflammatory reactions were characterised by qPCR and multiplex-ELISA. In total, the antibacterial effects of 23 AMPs were characterized. Only Tribolium castaneum Defensin 1 showed significant antibacterial effects against S. pneumoniae strain D39 and a multi-resistant clinical isolate. During in vitro infection of primary human macrophages with S. pneumoniae D39, Defensin 1 displayed strong antibacterial effects, and consequently reduced bacteria-induced cytokine expression and release. In summary, Tribolium castaneum Defensin 1 showed profound antibacterial effectivity against Streptococcus pneumoniae D39 and a multi-resistant clinical isolate without unwanted cytotoxic or inflammatory side effects on human blood-derived macrophages. |
| Databáze: | Directory of Open Access Journals |
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