Association of circulating microRNA‐122 and microRNA‐29a with stage of fibrosis and progression of chronic hepatitis in Labrador Retrievers

Autor: Manabu Sakai, Bart Spee, Guy C. M. Grinwis, Louis C. Penning, Monique E. vanWolferen, Luc J. W. van derLaan, Hille Fieten
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Zdroj: Journal of Veterinary Internal Medicine, Vol 33, Iss 1, Pp 151-157 (2019)
Druh dokumentu: article
ISSN: 1939-1676
0891-6640
DOI: 10.1111/jvim.15366
Popis: Background Chronic hepatitis (CH) in dogs is common and has the tendency to progress to liver cirrhosis (LC). Circulating microRNAs might have the potential as markers for disease progression. Objectives To investigate whether concentration of specific microRNAs in serum correlate with the stage and grade of CH in Labrador Retrievers. Animals Twenty‐two Labrador Retrievers with histological CH (n = 8), LC (n = 7), and normal liver (NL, n = 7). Methods In this retrospective study, serum concentrations of miR‐122, miR‐29a, miR‐133a, miR‐181b, and miR‐17‐5p were measured by quantitative real‐time PCR and evaluated using univariate linear regression in dogs. A multivariate model was fit including the grade of hepatitis and the stage of fibrosis. Results Of the 5 microRNAs, only circulating miR‐122 and miR‐29a were significantly associated with the grade of hepatitis and the stage of fibrosis. A positive correlation was identified between the grade of hepatitis with miR‐122 (rs = 0.79, P
Databáze: Directory of Open Access Journals
Externí odkaz: