Evaluation of antiradical assays used in determining the antioxidant capacity of pure compounds and plant extracts

Autor: Sandro de Oliveira, Glalci Alves de Souza, Camila Rodrigues Eckert, Thuany Alves Silva, Edmar Silva Sobral, Oriana Aparecida Fávero, Marcelo José Pena Ferreira, Paulete Romoff, Wilhelm Josef Baader
Jazyk: English<br />Spanish; Castilian<br />Portuguese
Rok vydání: 2014
Předmět:
Zdroj: Química Nova, Vol 37, Iss 3, Pp 497-503 (2014)
Druh dokumentu: article
ISSN: 1678-7064
0100-4042
DOI: 10.5935/0100-4042.20140076
Popis: The efficiency of the chemiluminescence luminol method and colorimetric DPPH and ABTS methods in evaluating the antiradical capacity of pure compounds and plant extracts with antioxidant potential is compared. In case of pure compounds, the values of parameter 'n' (number of radicals quenched per molecule of antiradical) for ascorbic acid, p-hydroquinone, catechol, quercetin, and rutin are similar when measured by colorimetric assays; however, considerably lower values of n are obtained with the luminol assay. The antiradical activity of extracts from male and female individuals of Baccharis burchelli and Baccharis crispa were determined by the luminol assay and expressed using the new Trolox® percentage (%Trolox®) parameter.
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