High Throughput Sequencing to Detect Differences in Methanotrophic Methylococcaceae and Methylocystaceae in Surface Peat, Forest Soil, and Sphagnum Moss in Cranesville Swamp Preserve, West Virginia, USA
| Autor: | Evan Lau, Edward J. Nolan IV, Zachary W. Dillard, Ryan D. Dague, Amanda L. Semple, Wendi L. Wentzell |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Methanotrophic bacteria
high throughput sequencing Illumina® MiSeq multiplex sequencing microbial ecology upland forest soils peatlands surface peat Sphagnum moss Methylococcaceae Methylocystaceae Evenness Shannon-Weiner Index Nonmetric multidimensional scaling biogeography Biology (General) QH301-705.5 |
| Zdroj: | Microorganisms, Vol 3, Iss 2, Pp 113-136 (2015) |
| Druh dokumentu: | article |
| ISSN: | 2076-2607 |
| DOI: | 10.3390/microorganisms3020113 |
| Popis: | Northern temperate forest soils and Sphagnum-dominated peatlands are a major source and sink of methane. In these ecosystems, methane is mainly oxidized by aerobic methanotrophic bacteria, which are typically found in aerated forest soils, surface peat, and Sphagnum moss. We contrasted methanotrophic bacterial diversity and abundances from the (i) organic horizon of forest soil; (ii) surface peat; and (iii) submerged Sphagnum moss from Cranesville Swamp Preserve, West Virginia, using multiplex sequencing of bacterial 16S rRNA (V3 region) gene amplicons. From ~1 million reads, >50,000 unique OTUs (Operational Taxonomic Units), 29 and 34 unique sequences were detected in the Methylococcaceae and Methylocystaceae, respectively, and 24 potential methanotrophs in the Beijerinckiaceae were also identified. Methylacidiphilum-like methanotrophs were not detected. Proteobacterial methanotrophic bacteria constitute |
| Databáze: | Directory of Open Access Journals |
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